Anti-HLA-DRB3 antibody produced in mouse
SIGMA/SAB1400125 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-HLA-DR3B; Anti-MGC117330
Product Type: Chemical
Western Blotting HLA-DRB3 polyclonal antibody. Western Blot analysis of HLA-DRB3 expression in human liver.
Western Blotting Western Blot analysis of HLA-DRB3 expression in transfected 293T cell line by HLA-DRB3 polyclonal antibody. Lanes Lane 1: HLA-DRB3 transfected lysate (29.26 kDa). Lane 2: Non-transfected lysate.
Flow Cytometry FACS analysis of negative control 293 cells (Black) and HLA-DRB3 expressing 293 cells (Green) using HLA-DRB3 purified mouse polyclonal antibody.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P79483  |
| General description: | HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB3 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. (provided by RefSeq) |
| Immunogen: | HLA-DRB3 (NP_072049.2, 1 a.a. ~ 266 a.a) full-length human protein. Sequence MVCLKLPGGSSLAALTVTLMVLSSR |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |