Monoclonal Anti-NID1 antibody produced in mouse
SIGMA/SAB1400185 - clone 1G3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ENTACTIN; Anti-NID; Anti-enactin
MDL Number: MFCD01865112
Product Type: Chemical
Western Blotting Western Blot analysis of NID1 expression in transfected 293T cell line by NID1 monoclonal antibody, clone 1G3. Lanes Lane 1: NID1 transfected lysate (122.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged NID1 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1G3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P14543  |
| General description: | This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. (provided by RefSeq) |
| Immunogen: | NID1 (NP_002499.1, 1148 a.a. ~ 1247 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EGLQYPFAVTSYGKNLYFTDWKMNS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |