Anti-VISA antibody produced in mouse
SIGMA/SAB1400655 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-CARDIF; Anti-DKFZp666M015; Anti-FLJ27482; Anti-FLJ41962; Anti-IPS-1; Anti-KIAA1271; Anti-MAVS
Product Type: Chemical
Western Blotting VISA polyclonal antibody. Western Blot analysis of VISA expression in human placenta.
Western Blotting Western Blot analysis of VISA expression in transfected 293T cell line by VISA polyclonal antibody. Lanes Lane 1: VISA transfected lysate (59.4 kDa). Lane 2: Non-transfected lysate.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1 μg/mL |
| General description: | Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 (MIM 603029) or by the cytoplasmic RNA helicases MDA5 (MIM 606951) and RIGI (ROBO3; MIM 608630). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK; MIM 600664) and IKKB (IKBKB; MIM 603258), which activate NFKB (see MIM 164011), or TBK1 (MIM 604834) and IKKE (IKBKE; MIM 605048), which activate IRF3 (MIM 603734). Activated IRF3 and NFKB induce transcription of IFNB (IFNB1; MIM 147640). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1; MIM 607601). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005 [PubMed 16239922]).[supplied by OMIM |
| Immunogen: | VISA (AAH44952.1, 1 a.a. ~ 540 a.a) full-length human protein. Sequence MPFAEDKTYKYICRNFSNFCNVDVV |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |