Anti-NXN antibody produced in mouse
SIGMA/SAB1400673 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-FLJ12614; Anti-TRG-4
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to NXN on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting NXN polyclonal antibody. Western Blot analysis of NXN expression in human kidney.
Western Blotting Western Blot analysis of NXN expression in transfected 293T cell line by NXN polyclonal antibody. Lanes Lane 1: NXN transfected lysate (47.85 kDa). Lane 2: Non-transfected lysate.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | Q6DKJ4  |
| Biochem/physiol Actions: | NXN (Nucleoredoxin) is associated with several regulatory cellular processes such as regulation of Wnt/β-catenin pathway, oxidative stress responses. It acts as a thiol reductase and reactive oxygen species regulator during thiol-mediated oxidoreduction process. NXN possesses specific binding affinity for protein phosphatase 2A (PP2A). It interacts directly to the catalytic subunit of PP2A during phosphatase activity. It has also been reported that NXN may be involved in the transcriptional regulation of NF-κβ, AP-1, and CREB activation in HEK293 cells. |
| General description: | NXN (Nucleoredoxin) is a novel cell growth regulatory protein belonging to the thioredoxin (TRX) family. It has a molecular mass of 55kDa. It is expressed in several adult tissues and is predominantly localized in the nucleus. It consists of a conserved TRX domain and a catalytic region, which exerts oxidoreductase activity. |
| Immunogen: | NXN (NP_071908, 1 a.a. ~ 435 a.a) full-length human protein. Sequence MSGFLEELLGEKLVTGGGEEVDVHS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |