Monoclonal Anti-CITED4 antibody produced in mouse
SIGMA/SAB1400832 - clone 4F6, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to CITED4 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to CITED4 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of CITED4 expression in transfected 293T cell line by CITED4 monoclonal antibody, clone 4F6. Lanes Lane 1: CITED4 transfected lysate (18.6 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (31.68 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q96RK1  |
| Biochem/physiol Actions: | CITED4 (Cbp (CREB-binding protein)/p300-interacting transactivator 4) is a transcriptional cofactor. It is found to be downregulated in some of the tumor types including colorectal cancer. It is involved in the regulation of TGFB (transforming growth factor β1), TFAP2 (transcription factor A) and HIF1A (hypoxia inducible factor 1 αsubunit). It prevents the transactivation of HIF1A by inhibiting its binding to p300. CITED4 is known to be involved in the development of neural crest and neural tube. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | CITED4 (Cbp (CREB-binding protein)/p300-interacting transactivator 4) gene is mapped to human chromosome 1p34.2. |
| Immunogen: | CITED4 (NP_597724.1, 131 a.a. ~ 184 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence GMDAELIDEEALTSLELELGLHRVR |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |