Anti-ADAR antibody produced in rabbit
SIGMA/SAB1401004 - purified immunoglobulin, buffered aqueous solution
Synonym: ADAR1; DRADA; DSH; DSRAD; G1P1; IFI-4
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to ADAR on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting ADAR rabbit polyclonal antibody. Western Blot analysis of ADAR expression in HeLa.
Western Blotting Western Blot analysis of ADAR expression in transfected 293T cell line by ADAR polyclonal antibody. Lanes Lane 1: ADAR transfected lysate (136.00 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | P55265   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P55265 |
| Application: | Anti-ADAR antibody produced in rabbit has been used in immunoprecipitation. |
| Biochem/physiol Actions: | Adenosine deaminase acting on RNA (ADAR) protein facilitates the conversion of adenosine to inosine in double-stranded RNAs (dsRNA). This protein is involved in the RNA interference pathway, to change the targeting and processing of microRNAs. Mutations in the ADAR gene lead to an auto-immune Aicardi-Goutières syndrome (AGS) and dyschromatosis symmetrica hereditarian (DSH). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Adenosine deaminase acting on RNA (ADAR) protein is an RNA -specific C-6 adenosine deaminase. This protein is composed of one or two N-terminal Z-DNA binding domains, three centrally located double-stranded RNA (dsRNA) binding domains, and a C-terminal deaminase catalytic domain. This protein has two isoforms present in several human cell lines. The ADAR gene is mapped on the human chromosome at 1q21.3. |
| General description: | This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. (provided by RefSeq) |
| Immunogen: | ADAR (P55265, 1 a.a. ~ 1226 a.a) full-length human protein. Sequence MNPRQGYSLSGYYTHPFQGYEHRQL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |