Monoclonal Anti-CD58 antibody produced in mouse
SIGMA/SAB1401059 - clone 3B12-D5, purified immunoglobulin, buffered aqueous solution
Synonym: LFA-3; LFA3
MDL Number: MFCD00675568
Product Type: Chemical
Western Blotting Western Blot analysis of CD58 expression in transfected 293T cell line by CD58 monoclonal antibody, clone 3B12-D5. Lanes Lane 1: CD58 transfected lysate (Predicted MW: 28.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (52.14 kDa).
ELISA Detection limit for recombinant GST tagged CD58 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3B12-D5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| NCBI accession no. | BC005930   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P19256 |
| General description: | This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. (provided by RefSeq) |
| Immunogen: | CD58 (AAH05930, 1 a.a. ~ 240 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MVAGSDAGRALGVLSVVCLLHCFGF |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |