Anti-HLA-E antibody produced in rabbit
SIGMA/SAB1401182 - purified immunoglobulin, buffered aqueous solution
Synonym: DKFZp686P19218; EA1.2; EA2.1; HLA-6.2; MHC; QA1
Product Type: Chemical
Western Blotting Western Blot analysis of HLA-E expression in transfected 293T cell line by HLA-E polyclonal antibody. Lanes Lane 1: HLA-E transfected lysate (40.20 kDa). Lane 2: Non-transfected lysate.
Immunoprecipitation Immunoprecipitation of HLA-E transfected lysate using anti-HLA-E rabbit polyclonal antibody and Protein A Magnetic Bead , and immunoblotted with HLA-E purified mouse polyclonal antibody (B01P) .
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | BC002578   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P13747 |
| General description: | HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. (provided by RefSeq) |
| Immunogen: | HLA-E (AAH02578.1, 1 a.a. ~ 358 a.a) full-length human protein. Sequence MVDGTLLLLLSEALALTQTWAGSHS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |