Monoclonal Anti-PPP2R1A antibody produced in mouse
SIGMA/SAB1401297 - clone 4E6, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between TP53 and PPP2R1A. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PPP2R1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting PPP2R1A monoclonal antibody, clone 4E6. Western Blot analysis of PPP2R1A expression in Jurkat.
ELISA Detection limit for recombinant GST tagged PPP2R1A is 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4E6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| NCBI accession no. | BC001537   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P30153 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. (provided by RefSeq) |
| Immunogen: | PPP2R1A (AAH01537, 1 a.a. ~ 589 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAAADGDDSLYPIAVLIDELRNEDV |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |