Monoclonal Anti-VAC14 antibody produced in mouse
SIGMA/SAB1401760 - clone 3B2, purified immunoglobulin, buffered aqueous solution
Synonym: ArPIKfyve; FLJ10305; FLJ36622; FLJ46582; MGC149815; MGC149816
Product Type: Chemical
Western Blotting Western Blot analysis of VAC14 expression in transfected 293T cell line by VAC14 monoclonal antibody, clone 3B2. Lanes Lane 1: VAC14 transfected lysate (Predicted MW: 88 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (33.33 kDa).
ELISA Detection limit for recombinant GST tagged VAC14 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3B2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| NCBI accession no. | NM_018052   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q08AM6 |
| General description: | Phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) is a low-abundance signaling molecule. A regulatory complex made up of VAC14 and FIG4 (MIM 609390) control synthesis of PI(3,5)P2 by activating PI(3)P kinase, FAB1 (PIP5K3; MIM 609414). The VAC14/FIG4 complex also functions in the breakdown of PI(3,5)P2 (Zhang et al., 2007 [PubMed 17956977]).[supplied by OMIM |
| Immunogen: | VAC14 (NP_060522.3, 714 a.a. ~ 782 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence SHRLQCVPNPELLQTEDSLKAAPKS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |