Monoclonal Anti-PUS1 antibody produced in mouse
SIGMA/SAB1401849 - clone 3A3, purified immunoglobulin, buffered aqueous solution
Synonym: MGC11268; MLASA
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PUS1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of PUS1 expression in transfected 293T cell line by PUS1 monoclonal antibody, clone 3A3. Lanes Lane 1: PUS1 transfected lysate (44.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (69.63 kDa).
Immunoprecipitation Immunoprecipitation of PUS1 transfected lysate using anti-PUS1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PUS1 rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3A3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| NCBI accession no. | BC002901   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunofluorescence: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9Y606 |
| General description: | This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants |
| Immunogen: | PUS1 (AAH02901, 1 a.a. ~ 399 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAGNAEPPPAGAACPQDRRSCSGRA |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |