Monoclonal Anti-HOOK3 antibody produced in mouse
SIGMA/SAB1401891 - clone 3A5, purified immunoglobulin, buffered aqueous solution
Synonym: FLJ31058; HK3
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to HOOK3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 μg/mL]
Western Blotting Western Blot analysis of HOOK3 expression in transfected 293T cell line by HOOK3 monoclonal antibody, clone 3A5. Lanes Lane 1: HOOK3 transfected lysate (Predicted MW: 83.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.41 kDa).
ELISA Detection limit for recombinant GST tagged HOOK3 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3A5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| NCBI accession no. | NM_032410   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q86VS8 |
| Biochem/physiol Actions: | In Caenorhabditis elegans, the HOOK3 (hook microtubule-tethering protein 3) protein is required for connecting the centrosome and the nucleus. It participates in transport of pericentriolar satellites, needed for centrosomal assembly in neurogenesis. HOOK3-RET (ret proto-oncogene) fusion protein is identified in papillary thyroid carcinoma and it results in tumor formation in nude mice. In presence of Salmonella enterica infection, the Salmonella SpiC (pathogenicity island 2 secreted effector protein) inactivates HOOK3 function and thereby affects the cellular trafficking and phagosome-lysosome fusion. |
| General description: | Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.[supplied by OMIM |
| Immunogen: | HOOK3 (NP_115786.1, 622 a.a. ~ 718 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence QNQGAAPEIQALKNQLQERDRLFHS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |