Monoclonal Anti-SLC13A5 antibody produced in mouse
SIGMA/SAB1402084 - clone 2G4, purified immunoglobulin, buffered aqueous solution
Synonym: DKFZp686E17257; MGC138356; NACT
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to SLC13A5 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 μg/mL]
Western Blotting SLC13A5 monoclonal antibody, clone 2G4. Western Blot analysis of SLC13A5 expression in HepG2.
Western Blotting QC Western Blot detection against Immunogen (31.79 kDa).
ELISA Detection limit for recombinant GST tagged SLC13A5 is 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2G4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| NCBI accession no. | NM_177550   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q86YT5 |
| Biochem/physiol Actions: | SLC13A5 (solute carrier family 13 member 5) is a sodium-coupled citrate transporter. Its function involves the transportation of citrate from the bloodstream into the liver. The expression of the gene is found to be increased in obesity and non-alcoholic fatty liver disease. Downregulation of SLC13A5 promotes AMPK (adenosine monophosphate-activated protein kinase) activation and inhibits ACLY (ATP citrate lyase) expression. SLC13A5 is thus found to regulate homeostasis of energy and lipid in liver cancer, as the gene is associated with synthesis of fatty acids and cholesterol. Mutation in the SLC13A5 gene leads to early occurrence of epilepsy, developmental delay and tooth dysplasia in children. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The solute carrier family 13 member 5 (SLC13A5) gene is mapped to human chromosome 17p13.1. It is highly expressed in the plasma membrane of hepatocytes and is also expressed in the cells of testis and brain. |
| Immunogen: | SLC13A5 (NP_808218, 152 a.a. ~ 206 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence VEAILQQMEATSAATEAGLELVDKG |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |