Anti-SPANXB1 antibody produced in mouse
SIGMA/SAB1402100 - purified immunoglobulin, buffered aqueous solution
Synonym: B1; SPANX-B; SPANXB
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to SPANXB1 on Hs 181.Tes cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of SPANXB1 expression in transfected 293T cell line by SPANXB1 polyclonal antibody. Lanes Lane 1: SPANXB1 transfected lysate (11.33 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NM_032461.2   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | Q9NS25 |
| General description: | Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene maps to chromosome X in a head-to-tail orientation with SPANX family member B2, which appears to be a duplication of the B1 locus. The SPANXB genes are unique members of this gene family, since they contain an additional 18 nt in their coding region compared to the majority of family members. Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis. (provided by RefSeq) |
| Immunogen: | SPANXB1 (NP_115850.1, 1 a.a. ~ 103 a.a) full-length human protein. Sequence MGQQSSVRRLKRSVPCESNEANEAN |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |