Monoclonal Anti-ACAT2 antibody produced in mouse
SIGMA/SAB1402106 - clone 4A5, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD08064410
Product Type: Chemical
Western Blotting Western Blot analysis of ACAT2 expression in transfected 293T cell line by ACAT2 monoclonal antibody, clone 4A5. Lanes Lane 1: ACAT2 transfected lysate (41.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (69.41 kDa).
ELISA Detection limit for recombinant GST tagged ACAT2 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4A5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~69.78 kDa |
| NCBI accession no. | BC000408   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9BWD1 |
| Biochem/physiol Actions: | ACAT2 (acetyl-CoA acetyltransferase 2) is responsible for the production of cholesteryl esters from cholesterol and fatty acids. In mouse model, absence of intestinal or hepatic ACAT2 results in atheroprotective effects. Thus, it is suggested as a target for treatment of hypercholesterolemia. Mutations in this gene are associated with changes in plasma lipid levels and coronary artery disease (CAD) risk. |
| General description: | The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. (provided by RefSeq) |
| Immunogen: | ACAT2 (AAH00408, 1 a.a. ~ 397 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MNAGSDPVVIVSAARTIIGSFNGAL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |