Monoclonal Anti-NR0B1, (C-terminal) antibody produced in mouse
SIGMA/SAB1402110 - clone 1F10, purified immunoglobulin, buffered aqueous solution
Synonym: AHC; AHCH; AHX; DAX-1; DAX1; DSS; GTD; HHG; NROB1
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to NR0B1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting NR0B1 monoclonal antibody, clone 1F10 Western Blot analysis of NR0B1 expression in HeLa S3 NE.
ELISA Detection limit for recombinant GST tagged NR0B1 is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1F10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG3κ |
| mol wt | antigen ~38.21 kDa |
| NCBI accession no. | NM_000475   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| direct immunofluorescence: suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P51843 |
| Biochem/physiol Actions: | The encoded orphan nuclear receptor is involved in the growth and activity of the adrenal and reproductive organs. It is significantly associated with the growth of gonads, development of sexual characteristics and steroidogenesis. The expression of NR0B1 (nuclear receptor subfamily 0 group B member 1) gene is regulated by Nanog (transcription factor). NR0B1 is known to control pluripotency and differentiation of embryonic stem cells in mice. Mutation in NR0B1 is importantly associated with X-linked adrenal hypoplasia congenita. Mutations in NR0B1 is also known to downregulate the androgen receptor activity, also leading to defect in spermatogenesis. |
| General description: | This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. (provided by RefSeq) |
| Immunogen: | NR0B1 (NP_000466, 361 a.a. ~ 470 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence IKCFLSKCWSLNISTKEYAYLKGTV |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |