Monoclonal Anti-BDNF antibody produced in mouse
SIGMA/SAB1402127 - clone 1B10, purified immunoglobulin, buffered aqueous solution
Synonym: MGC34632
MDL Number: MFCD01633045
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to BDNF on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of BDNF expression in transfected 293T cell line by BDNF monoclonal antibody, clone 1B10. Lanes Lane 1: BDNF transfected lysate (28.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting BDNF monoclonal antibody, clone 1B10. Western Blot analysis of BDNF expression in IMR-32.
Western Blotting QC Western Blot detection against Immunogen (52.91 kDa).
Immunoprecipitation Immunoprecipitation of BDNF transfected lysate using anti-BDNF monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with BDNF monoclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1B10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~53.28 kDa |
| NCBI accession no. | BC029795   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P23560 |
| Application: | Monoclonal Anti-BDNF antibody produced in mouse has been used in western blotting. |
| Biochem/physiol Actions: | Brain derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. Variation in the BDNF gene expression alters these neurological functions. BDNF also plays a vital role in vascular function and participates in angiogenesis via the specific receptor tropomyosin-related kinase B (TrkB). ProBDNF interacts with the p75 neurotrophin receptor, leading to long-term depression in the hippocampus. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The brain derived neurotrophic factor (BDNF) gene is mapped to human chromosome 11p14.1. |
| General description: | The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer′s and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. (provided by RefSeq) |
| Immunogen: | BDNF (AAH29795, 1 a.a. ~ 247 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MTILFLTMVISYFGCMKAAPMKEAN |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |