Monoclonal Anti-COL1A1 antibody produced in mouse
SIGMA/SAB1402151 - clone 3G3, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to COL1A1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 μg/mL]
Western Blotting COL1A1 monoclonal antibody, clone 3G3. Western Blot analysis of COL1A1 expression in NIH/3T3.
Western Blotting QC Western Blot detection against Immunogen (35.42 kDa).
ELISA Detection limit for recombinant GST tagged COL1A1 is 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3G3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG3κ |
| mol wt | antigen ~35.79 kDa |
| NCBI accession no. | NM_000088   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P02452 |
| Biochem/physiol Actions: | Proteins belonging to the collagen family, supports and nourishes body tissues such as tendons, cartilage, skin, and sclera of the eye. Mutation in COL1A1 (collagen type I α 1 chain) gene is associated with a number of connective tissue disorders such as Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, osteogenesis imperfecta types I-IV, idiopathic osteoporosis and Caffey Disease. The COL1A1 is associated with a particular type of skin tumor called dermatofibrosarcoma protuberans which is due to over expression of growth factors. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The COL1A1 (collagen type I α 1 chain) gene codes for the pro-α1 chains of type I collagen. The triple helix of the type I collagen is composed of two α chains and this protein is localized to connective tissues and predominantly expressed in bone, cornea, dermis and tendon. COL1A1 is mapped to human chromosome 17q21.33. 90% of the bone matrix comprises of collagen type I. |
| Immunogen: | COL1A1 (NP_000079.1, 1021 a.a. ~ 1108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EGSPGRDGSPGAKGDRGETGPAGPP |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |