Monoclonal Anti-CPA1 antibody produced in mouse
SIGMA/SAB1402153 - clone 3F11, purified immunoglobulin, buffered aqueous solution
Synonym: CPA
Product Type: Chemical
Western Blotting Western Blot analysis of CPA1 expression in transfected 293T cell line by CPA1 monoclonal antibody, clone 3F11. Lanes Lane 1: CPA1 transfected lysate (47.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (71.83 kDa).
Immunoprecipitation Immunoprecipitation of CPA1 transfected lysate using anti-CPA1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with CPA1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged CPA1 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~71.83 kDa |
| NCBI accession no. | BC005279   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P15085 |
| Biochem/physiol Actions: | CPA1 (Carboxypeptidase A1) is a zinc metalloenzyme belonging to the tetragonal space group P4(3)2(1)2. It forms a multiprotein complex, Zn2+-poly(acrylic acid), by directly interacting with proteases in the gastrointestinal tract. It stimulates the hydrolytic separation of carboxyl-terminal aromatic or branched aliphatic side chain of amide bonds from peptides and proteins. |
| General description: | Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a monomeric pancreatic exopeptidase involved in zymogen inhibition. (provided by RefSeq) |
| Immunogen: | CPA1 (AAH05279, 1 a.a. ~ 419 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MRGLLVLSVLLGAVFGKEDFVGHQV |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |