Monoclonal Anti-EPB42, (C-terminal) antibody produced in mouse
SIGMA/SAB1402184 - clone 2G12, purified immunoglobulin, buffered aqueous solution
Synonym: MGC116735; MGC116737; PA
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to EPB42 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of EPB42 expression in transfected 293T cell line by EPB42 monoclonal antibody, clone 2G12. Lanes Lane 1: EPB42 transfected lysate (69.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged EPB42 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2G12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~37 kDa |
| NCBI accession no. | NM_000119   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P16452 |
| General description: | Erythrocyte membrane protein band 4.2 is an ATP-binding protein which may regulate the association of protein 3 with ankyrin. It probably has a role in erythrocyte shape and mechanical property regulation. Mutations in the EPB42 gene are associated with recessive spherocytic elliptocytosis and recessively transmitted hereditary hemolytic anemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | EPB42 (NP_000110.1, 623 a.a. ~ 721 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KMPEKAEQYQPLTASVSLQNSLDAP |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |