Monoclonal Anti-GIF, (C-terminal) antibody produced in mouse
SIGMA/SAB1402207 - clone 1D9, purified immunoglobulin, buffered aqueous solution
Synonym: IF; IFMH; INF; TCN3
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to GIF on HeLa cell. [antibody concentration 10 μg/mL]
Immunoprecipitation Immunoprecipitation of GIF transfected lysate using anti-GIF monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with GIF rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged GIF is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~37.11 kDa |
| NCBI accession no. | NM_005142   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunofluorescence: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| UniProt accession no. | P27352 |
| General description: | This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. (provided by RefSeq) |
| Immunogen: | GIF (NP_005133.2, 318 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence INNQLRGVELLFNETINVSVKSGSV |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |