Monoclonal Anti-HNRPA1 antibody produced in mouse
SIGMA/SAB1402226 - clone 2E6, purified immunoglobulin, buffered aqueous solution
Synonym: HNRPA1; MGC102835
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to HNRNPA1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of HNRNPA1 expression in transfected 293T cell line by HNRPA1 monoclonal antibody, clone 2E6. Lanes Lane 1: HNRNPA1 transfected lysate (34.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (60.94 kDa).
ELISA Detection limit for recombinant GST tagged HNRNPA1 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~61.31 kDa |
| NCBI accession no. | BC033714   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P09651 |
| General description: | This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites. (provided by RefSeq) |
| Immunogen: | HNRPA1 (AAH33714.1, 1 a.a. ~ 320 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSKSESPKEPEQLRKLFIGGLSFET |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |