Monoclonal Anti-MAGEB1 antibody produced in mouse
SIGMA/SAB1402262 - clone 2A4, purified immunoglobulin, buffered aqueous solution
Synonym: DAM10; MAGE-Xp; MAGEL1; MGC9322
Product Type: Chemical
Western Blotting Western Blot analysis of MAGEB1 expression in transfected 293T cell line by MAGEB1 monoclonal antibody, clone 2A4. Lanes Lane 1: MAGEB1 transfected lysate (Predicted MW: 39 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (63.91 kDa).
ELISA Detection limit for recombinant GST tagged MAGEB1 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2A4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~64.28 kDa |
| NCBI accession no. | BC013772   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P43366 |
| General description: | This gene is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region, and expressed in testis and in a significant fraction of tumors of various histological types. This gene and other MAGEB members are clustered on chromosome Xp22-p21. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene, however, the full length nature of some variants has not been defined. (provided by RefSeq) |
| Immunogen: | MAGEB1 (AAH13772, 1 a.a. ~ 347 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MPRGQKSKLRAREKRRKAREETQGL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |