Monoclonal Anti-MARK3 antibody produced in mouse
SIGMA/SAB1402263 - clone 1A10, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD03454919
Product Type: Chemical
Western Blotting Western Blot analysis of MARK3 expression in transfected 293T cell line by MARK3 monoclonal antibody, clone 1A10. Lanes Lane 1: MARK3 transfected lysate (81.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged MARK3 is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1A10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2bκ |
| mol wt | antigen ~37 kDa |
| NCBI accession no. | NM_002376   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P27448 |
| Biochem/physiol Actions: | MARK3 (microtubule affinity regulating kinase 3) is activated by phosphorylation and is responsible for the phosphorylation of other proteins. Changes in the expression of MARK3 are associated with carcinogenesis. |
| Immunogen: | MARK3 (NP_002367.4, 402 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence HHKVQRSVFSSQKQRRYSDHAGPAI |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |