Monoclonal Anti-MB antibody produced in mouse
SIGMA/SAB1402265 - clone 4F8, purified immunoglobulin, buffered aqueous solution
Synonym: MGC13548; PVALB
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to MB on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of MB expression in transfected 293T cell line by MB monoclonal antibody, clone 4F8. Lanes Lane 1: MB transfected lysate (17.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.52 kDa).
ELISA Detection limit for recombinant GST tagged MB is 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~36.89 kDa |
| NCBI accession no. | NM_005368.2   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P02144 |
| General description: | This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. (provided by RefSeq) |
| Immunogen: | MB (NP_005359.1, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence GLSDGEWQLVLNVWGKVEADIPGHG |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |