Monoclonal Anti-MAP3K11, (C-terminal) antibody produced in mouse
SIGMA/SAB1402271 - clone 3D11, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to MAP3K11 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between CDC42 and MAP3K11. HeLa cells were stained with anti-CDC42 rabbit purified polyclonal 1:1200 and anti-MAP3K11 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting Western Blot analysis of MAP3K11 expression in transfected 293T cell line by MAP3K11 monoclonal antibody, clone 3D11. Lanes Lane 1: MAP3K11 transfected lysate (92.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.51 kDa).
ELISA Detection limit for recombinant GST tagged MAP3K11 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3D11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~37.51 kDa |
| NCBI accession no. | NM_002419   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q16584 |
| General description: | The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. (provided by RefSeq) |
| Immunogen: | MAP3K11 (NP_002410, 741 a.a. ~ 847 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PPPGTSRSAPGTPGTPRSPPLGLIS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |