Monoclonal Anti-NDUFA8, (N-terminal) antibody produced in mouse
SIGMA/SAB1402285 - clone 2E10, purified immunoglobulin, buffered aqueous solution
Synonym: CI-19KD; CI-PGIV; MGC793; PGIV
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to NDUFA8 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 μg/mL]
Western Blotting Western Blot analysis of NDUFA8 expression in transfected 293T cell line by NDUFA8 monoclonal antibody, clone 2E10. Lanes Lane 1: NDUFA8 transfected lysate (Predicted MW: 20.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (33.66 kDa).
ELISA Detection limit for recombinant GST tagged NDUFA8 is 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~34.03 kDa |
| NCBI accession no. | NM_014222   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P51970 |
| General description: | The protein encoded by this gene belongs to the complex I 19 kDA subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. (provided by RefSeq) |
| Immunogen: | NDUFA8 (NP_055037.1, 1 a.a. ~ 72 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MPGIVELPTLEELKVDEVKISSAVL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |