Monoclonal Anti-PRF1, (C-terminal) antibody produced in mouse
SIGMA/SAB1402323 - clone 3B4, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Western Blotting Western Blot analysis of PRF1 expression in transfected 293T cell line by PRF1 monoclonal antibody, clone 3B4. Lanes Lane 1: PRF1 transfected lysate (61.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.19 kDa).
Immunoprecipitation Immunoprecipitation of PRF1 transfected lysate using anti-PRF1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PRF1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged PRF1 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3B4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~36.56 kDa |
| NCBI accession no. | NM_005041   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P14222 |
| General description: | The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. (provided by RefSeq) |
| Immunogen: | PRF1 (NP_005032.2, 461 a.a. ~ 555 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence WSVRLDFGDVLLATGGPLRLQVWDQ |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |