Monoclonal Anti-SGCA antibody produced in mouse
SIGMA/SAB1402353 - clone 3C4, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Western Blotting Western Blot analysis of SGCA expression in transfected 293T cell line by SGCA monoclonal antibody, clone 3C4. Lanes Lane 1: SGCA transfected lysate (42.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.62 kDa).
ELISA Detection limit for recombinant GST tagged SGCA is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3C4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2bκ |
| mol wt | antigen ~37.99 kDa |
| NCBI accession no. | NM_000023   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q16586 |
| General description: | This gene encodes a component of the dystrophin-glycoprotein complex (DGC), which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | SGCA (NP_000014.1, 26 a.a. ~ 133 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence TTLHPLVGRVFVHTLDHETFLSLPE |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |