Monoclonal Anti-USO1 antibody produced in mouse
SIGMA/SAB1402428 - clone 3F4, purified immunoglobulin, buffered aqueous solution
Synonym: P115; TAP; VDP
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to USO1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to USO1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting USO1 monoclonal antibody, clone 3F4. Western Blot analysis of USO1 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (131.45 kDa).
ELISA Detection limit for recombinant GST tagged USO1 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~131.82 kDa |
| NCBI accession no. | BC032654   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O60763 |
| General description: | The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner. (provided by RefSeq) |
| Immunogen: | USO1 (AAH32654, 1 a.a. ~ 961 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MNFLRGVMGGQSAGPQHTEAETIQK |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |