Monoclonal Anti-EDF1 antibody produced in mouse
SIGMA/SAB1402430 - clone 3E6, purified immunoglobulin, buffered aqueous solution
Synonym: EDF-1; MBF1; MGC9058
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to EDF1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to EDF1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting EDF1 monoclonal antibody, clone 3E6. Western Blot analysis of EDF1 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (42.02 kDa).
ELISA Detection limit for recombinant GST tagged EDF1 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3E6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~42.39 kDa |
| NCBI accession no. | BC015500   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O60869 |
| General description: | This gene encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene. (provided by RefSeq) |
| Immunogen: | EDF1 (AAH15500.1, 1 a.a. ~ 148 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAESDWDTVTVLRKKGPTAAQAKSK |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |