Monoclonal Anti-EIF2S2, (N-terminal) antibody produced in mouse
SIGMA/SAB1402439 - clone 2F3, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to EIF2S2 on formalin-fixed paraffin-embedded human pancreatic cancer. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to EIF2S2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting EIF2S2 monoclonal antibody, clone 2F3. Western Blot analysis of EIF2S2 expression in Raw 264.7.
Western Blotting EIF2S2 monoclonal antibody, clone 2F3. Western Blot analysis of EIF2S2 expression in NIH/3T3.
Western Blotting EIF2S2 monoclonal antibody, clone 2F3. Western Blot analysis of EIF2S2 expression in HeLa.
Western Blotting EIF2S2 monoclonal antibody, clone 2F3. Western Blot analysis of EIF2S2 expression in PC-12.
Western Blotting Western Blot analysis of EIF2S2 expression in transfected 293T cell line by EIF2S2 monoclonal antibody, clone 2F3. Lanes Lane 1: EIF2S2 transfected lysate (38.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged EIF2S2 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~37.11 kDa |
| NCBI accession no. | NM_003908   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P20042 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. (provided by RefSeq) |
| Immunogen: | EIF2S2 (NP_003899.2, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSGDEMIFDPTMSKKKKKKKKPFML |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |