Monoclonal Anti-EIF1AY antibody produced in mouse
SIGMA/SAB1402445 - clone 1B4, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to EIF1AY on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting EIF1AY monoclonal antibody, clone 1B4. Western Blot analysis of EIF1AY expression in HeLa.
Western Blotting Western Blot analysis of EIF1AY expression in transfected 293T cell line by EIF1AY monoclonal antibody, clone 1B4. Lanes Lane 1: EIF1AY transfected lysate (16.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (33.88 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1B4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~34.65 kDa |
| NCBI accession no. | BC005248   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O14602 |
| General description: | This gene encodes a protein similar to eukaryotic translation initiation factor 1A (EIF1A). EIF1A is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5′ end of capped RNA. (provided by RefSeq) |
| Immunogen: | EIF1AY (NP_004672.2, 22 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EKRELVFKEDGQEYAQVIKMLGNGR |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |