Monoclonal Anti-ARHGEF6 antibody produced in mouse
SIGMA/SAB1402473 - clone 2E5, purified immunoglobulin, buffered aqueous solution
Synonym: COOL2; Cool-2; KIAA0006; MRX46; PIXA; alpha-PIX; alphaPIX
Product Type: Chemical
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between CDC42 and ARHGEF6. HeLa cells were stained with anti-CDC42 rabbit purified polyclonal 1:1200 and anti-ARHGEF6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting Western Blot analysis of ARHGEF6 expression in transfected 293T cell line by ARHGEF6 monoclonal antibody, clone 2E5. Lanes Lane 1: ARHGEF6 transfected lysate (Predicted MW: 87.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (38.06 kDa).
Immunoprecipitation Immunoprecipitation of ARHGEF6 transfected lysate using anti-ARHGEF6 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with ARHGEF6 monoclonal antibody.
ELISA Detection limit for recombinant GST tagged ARHGEF6 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~38.65 kDa |
| NCBI accession no. | BC039856   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q15052 |
| General description: | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific mental retardation. (provided by RefSeq) |
| Immunogen: | ARHGEF6 (AAH39856.1, 61 a.a. ~ 175 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence QTEADCINNINDFLKGCATLQVEIF |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |