Monoclonal Anti-RAPGEF5 antibody produced in mouse
SIGMA/SAB1402485 - clone 2E4, purified immunoglobulin, buffered aqueous solution
Synonym: GFR; KIAA0277; MR-GEF; REPAC
Product Type: Chemical
Western Blotting RAPGEF5 monoclonal antibody, clone 2E4. Western Blot analysis of RAPGEF5 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (74.58 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~74.95 kDa |
| NCBI accession no. | BC039203   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q92565 |
| General description: | Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM |
| Immunogen: | RAPGEF5 (AAH39203, 1 a.a. ~ 444 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGSSRLRVFDPHLERKDSAAALSDR |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |