Monoclonal Anti-NTE, (C-terminal) antibody produced in mouse
SIGMA/SAB1402533 - clone 3D10, purified immunoglobulin, buffered aqueous solution
Synonym: NTE; NTEMND; SPG39; sws
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to PNPLA6 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 μg/mL]
Western Blotting Western Blot analysis of PNPLA6 expression in transfected 293T cell line by NTE monoclonal antibody, clone 3D10. Lanes Lane 1: PNPLA6 transfected lysate (Predicted MW: 146.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged PNPLA6 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3D10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~37 kDa |
| NCBI accession no. | NM_006702   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q8IY17 |
| Immunogen: | NTE (NP_006693.2, 1229 a.a. ~ 1327 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence ESRRADVLAFPSSGFTDLAEIVSRI |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |