Monoclonal Anti-ACOT7, (N-terminal) antibody produced in mouse
SIGMA/SAB1402541 - clone 1E7, purified immunoglobulin, buffered aqueous solution
Synonym: ACH1; ACT; BACH; CTE-II; LACH; LACH1; MGC1126; hBACH
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to ACOT7 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 μg/mL]
Western Blotting Western Blot analysis of ACOT7 expression in transfected 293T cell line by ACOT7 monoclonal antibody, clone 1E7. Lanes Lane 1: ACOT7 transfected lysate (Predicted MW: 41.8 kDa). Lane 2: Non-transfected lysate.
Western Blotting ACOT7 monoclonal antibody, clone 1E7. Western Blot analysis of ACOT7 expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (62.92 kDa).
ELISA Detection limit for recombinant GST tagged ACOT7 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1E7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~63.29 kDa |
| NCBI accession no. | BC017365   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O00154 |
| Biochem/physiol Actions: | ACOT7 (acyl-CoA thioesterase 7) is involved in fatty acid oxidation. It is a connecting link between metabolism of acyl-CoAs and cholesterol in neurons. Overexpression of this gene in macrophage cell line modulates the generation of prostaglandins D2 and E2, thereby making it a drug target in inflammatory diseases. ACOT7 has high specificity for arachido-258 noyl-CoA (AA-CoA). It might also be associated with mesial temporal lobe epilepsy (MTLE). |
| General description: | This gene encodes a member of the acyl coenzyme family. The encoded protein hydrolyzes the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Decreased expression of this gene may be associated with mesial temporal lobe epilepsy. Alternatively spliced transcript variants encoding distinct isoforms with different subcellular locations have been characterized. (provided by RefSeq) |
| Immunogen: | ACOT7 (AAH17365, 1 a.a. ~ 338 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSGPDVETPSAIQICRIMRPDDANV |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |