Monoclonal Anti-RNF25, (C-terminal) antibody produced in mouse
SIGMA/SAB1402623 - clone 1D10, purified immunoglobulin, buffered aqueous solution
Synonym: AO7; FLJ13906
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to RNF25 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 μg/mL]
Western Blotting QC Western Blot detection against Immunogen (37.62 kDa).
ELISA Detection limit for recombinant GST tagged RNF25 is 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~37.99 kDa |
| NCBI accession no. | NM_022453   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q96BH1 |
| General description: | The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination. (provided by RefSeq) |
| Immunogen: | RNF25 (NP_071898.2, 352 a.a. ~ 459 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence RRHPKGGECHAPKGTRDTQELPPPE |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |