Monoclonal Anti-PDZD3 antibody produced in mouse
SIGMA/SAB1402627 - clone 1F2, purified immunoglobulin, buffered aqueous solution
Synonym: FLJ22756; IKEPP; PDZK2
Product Type: Chemical
Western Blotting Western Blot analysis of PDZD3 expression in transfected 293T cell line by PDZD3 monoclonal antibody, clone 1F2. Lanes Lane 1: PDZD3 transfected lysate (52.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (79.53 kDa).
ELISA Detection limit for recombinant GST tagged PDZD3 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1F2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~80.12 kDa |
| NCBI accession no. | BC029042   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q86UT5 |
| General description: | Guanylyl cyclase C (GCC, or GUCY2C; MIM 601330) produces cGMP following the binding of either endogenous ligands or heat-stable enterotoxins secreted by E. coli and other enteric bacteria. Activation of GCC initiates a signaling cascade that leads to phosphorylation of the cystic fibrosis transmembrane conductance regulator (CFTR; MIM 602421), followed by a net efflux of ions and water into the intestinal lumen. IKEPP is a regulatory protein that associates with GCC and regulates the amount of cGMP produced following receptor stimulation (Scott et al., 2002 [PubMed 11950846]).[supplied by OMIM |
| Immunogen: | PDZD3 (AAH29042, 1 a.a. ~ 491 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MEKAADLQDTASLTLKFKFNPKLGI |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |