Monoclonal Anti-PHACTR3 antibody produced in mouse
SIGMA/SAB1402658 - clone 4A5, purified immunoglobulin, buffered aqueous solution
Synonym: C20orf101; H17739; MGC117178; SCAPIN1; SCAPININ
Product Type: Chemical
Western Blotting Western Blot analysis of PHACTR3 expression in transfected 293T cell line by PHACTR3 monoclonal antibody, clone 4A5. Lanes Lane 1: PHACTR3 transfected lysate (51.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (35.42 kDa).
ELISA Detection limit for recombinant GST tagged PHACTR3 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4A5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~35.79 kDa |
| NCBI accession no. | NM_080672   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q96KR7 |
| General description: | The protein encoded by this gene is associated with the nuclear scaffold in proliferating cells. It was found to bind to the catalytic subunit of protein phosphatase-1 (PP1) and inhibit PP1 activity, suggesting that this protein may function as a regulatory subunit of PP1. Alternative splicing at this locus results in several transcript variants encoding different isoforms. (provided by RefSeq) |
| Immunogen: | PHACTR3 (NP_542403.1, 92 a.a. ~ 179 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence TSALEKKMAGRQGREELIKKGLLEM |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |