Monoclonal Anti-NUP43, (C-terminal) antibody produced in mouse
SIGMA/SAB1402675 - clone 2G5, purified immunoglobulin, buffered aqueous solution
Synonym: FLJ13287; bA350J20.1; p42
Product Type: Chemical
Western Blotting Western Blot analysis of NUP43 expression in transfected 293T cell line by NUP43 monoclonal antibody, clone 2G5. Lanes Lane 1: NUP43 transfected lysate (42.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting NUP43 monoclonal antibody, clone 2G5. Western Blot analysis of NUP43 expression in human colon.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged NUP43 is 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2G5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~37.11 kDa |
| NCBI accession no. | NM_024647   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q8NFH3 |
| General description: | Bidirectional transport of macromolecules between the cytoplasm and nucleus occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. NPCs are composed of subcomplexes, and NUP43 is part of one such subcomplex, Nup107-160 (Loiodice et al., 2004 [PubMed 15146057]).[supplied by OMIM |
| Immunogen: | NUP43 (NP_078923.3, 281 a.a. ~ 380 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence SEDGSLWHWDASTDVPEKSSLFHQG |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |