Monoclonal Anti-BCAT1 antibody produced in mouse
SIGMA/SAB1402701 - clone 1F8, purified immunoglobulin, buffered aqueous solution
Synonym: BCT1; DKFZp686E12175; ECA39; MECA39; PNAS-121; PP18
Product Type: Chemical
Western Blotting BCAT1 monoclonal antibody, clone 1F8. Western Blot analysis of BCAT1 expression in MCF-7.
Western Blotting BCAT1 monoclonal antibody, clone 1F8. Western Blot analysis of BCAT1 expression in human kidney.
ELISA Detection limit for recombinant GST tagged BCAT1 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1F8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~61.31 kDa |
| NCBI accession no. | BC033864   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P54687 |
| General description: | This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemi |
| Immunogen: | BCAT1 (AAH33864, 1 a.a. ~ 320 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDCSNGCSAECTGEGGSKEVVGTFK |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |