Monoclonal Anti-C4BPB antibody produced in mouse
SIGMA/SAB1402704 - clone 1G9, purified immunoglobulin, buffered aqueous solution
Synonym: C4BP
Product Type: Chemical
Western Blotting QC Western Blot detection against Immunogen (53.35 kDa).
Immunoprecipitation Immunoprecipitation of C4BPB transfected lysate using anti-C4BPB monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with C4BPB rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged C4BPB is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1G9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~53.72 kDa |
| NCBI accession no. | BC005378   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P20851 |
| General description: | This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. A single, unique beta-chain encoded by this gene assembles with seven identical alpha-chains into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. C4b-binding protein has a regulatory role in the coagulation system also, mediated through the beta-chain binding of protein S, a vitamin K-dependent protein that serves as a cofactor of activated protein C. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Alternative splicing gives rise to multiple transcript variants. (provided by RefSeq) |
| Immunogen: | C4BPB (AAH05378, 1 a.a. ~ 251 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MFFWCACCLMVAWRVSASDEHCPEL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |