Monoclonal Anti-COX15 antibody produced in mouse
SIGMA/SAB1402734 - clone 2D2, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Western Blotting Western Blot analysis of COX15 expression in transfected 293T cell line by COX15 monoclonal antibody, clone 2D2. Lanes Lane 1: COX15 transfected lysate (Predicted MW: 46 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (32.45 kDa).
ELISA Detection limit for recombinant GST tagged COX15 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2D2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~32.82 kDa |
| NCBI accession no. | NM_078470   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q7KZN9 |
| General description: | Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be essential for the biogenesis of COX formation and may function in the hydroxylation of heme O, according to the yeast mutant studies. This protein is predicted to contain 5 transmembrane domains localized in the mitochondrial inner membrane. Alternative splicing of this gene generates two transcript variants diverging in the 3′ region. (provided by RefSeq) |
| Immunogen: | COX15 (NP_510870, 92 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LTESGLSMVDWHLIKEMKPPTSQEE |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |