Monoclonal Anti-CRABP2 antibody produced in mouse
SIGMA/SAB1402736 - clone 4F2, purified immunoglobulin, buffered aqueous solution
Synonym: CRABP-II; RBP6
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to CRABP2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of CRABP2 expression in transfected 293T cell line by CRABP2 monoclonal antibody, clone 4F2. Lanes Lane 1: CRABP2 transfected lysate (15.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (42.1 kDa).
ELISA Detection limit for recombinant GST tagged CRABP2 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~42.7 kDa |
| NCBI accession no. | NM_001878   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P29373 |
| Application: | Monoclonal Anti-CRABP2 antibody produced in mouse is suitable for indirect ELISA and western blot applications. |
| Biochem/physiol Actions: | Cellular retinoic acid binding protein 2 (CRABP2) plays a crucial role in acceleration of the transcriptional activity. In the nucleus, by binding directly to the nuclear receptor RAR, it forms a complex for connecting retinoic acid (RA) from the binding protein to the receptor. This interaction finally helps to accelerate the transcriptional activity. It may have impact in the immature phenotype in glioma cells. |
| General description: | A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The inducibility of the CRABP2 gene suggests that this isoform is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein. (provided by RefSeq) |
| Immunogen: | CRABP2 (NP_001869.1, 1 a.a. ~ 138 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MPNFSGNWKIIRSENFEELLKVLGV |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |