Monoclonal Anti-PHC2 antibody produced in mouse
SIGMA/SAB1402759 - clone 1F4, purified immunoglobulin, buffered aqueous solution
Synonym: EDR2; HPH2; MGC163502; PH2
Product Type: Chemical
Western Blotting Western Blot analysis of PHC2 expression in transfected 293T cell line by PHC2 monoclonal antibody, clone 1F4. Lanes Lane 1: PHC2 transfected lysate (Predicted MW: 35.8 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1F4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~38.21 kDa |
| NCBI accession no. | NM_004427   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q8IXK0 |
| General description: | In Drosophila melanogaster, the ′Polycomb′ group (PcG) of genes are part of a cellular memory system that is responsible for the stable inheritance of gene activity. PcG proteins form a large multimeric, chromatin-associated protein complex. The protein encoded by this gene has homology to the Drosophila PcG protein ′polyhomeotic′ (Ph) and is known to heterodimerize with EDR1 and colocalize with BMI1 in interphase nuclei of human cells. The specific function in human cells has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | PHC2 (NP_004418.2, 91 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PYLQESKEEGAPLKLKCELCGRVDF |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |