Monoclonal Anti-EIF5A antibody produced in mouse
SIGMA/SAB1402762 - clone 8C1, purified immunoglobulin, buffered aqueous solution
Synonym: EIF-5A; EIF5A1; MGC104255; MGC99547; eIF5AI
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to EIF5A on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of EIF5A expression in transfected 293T cell line by EIF5A monoclonal antibody, clone 8C1. Lanes Lane 1: EIF5A transfected lysate (16.8 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (43.2 kDa).
ELISA Detection limit for recombinant GST tagged EIF5A is 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8C1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~43.8 kDa |
| NCBI accession no. | NM_001970   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunofluorescence: suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P63241 |
| Immunogen: | EIF5A (NP_001961.1, 1 a.a. ~ 154 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MADDLDFETGDAGASATFPMQCSAL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |