Monoclonal Anti-GLE1 antibody produced in mouse
SIGMA/SAB1402789 - clone 1D8, purified immunoglobulin, buffered aqueous solution
Synonym: GLE1L; LCCS; LCCS1; hGLE1
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to GLE1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting QC Western Blot detection against Immunogen (36.85 kDa).
ELISA Detection limit for recombinant GST tagged GLE1 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~37.22 kDa |
| NCBI accession no. | NM_001003722   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunofluorescence: suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q53GS7 |
| General description: | This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | GLE1 (NP_001003722.1, 140 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence RMKGTEGLRLWQEEQERKVQALSEM |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |