Monoclonal Anti-HOXD1 antibody produced in mouse
SIGMA/SAB1402809 - clone 4F4, purified immunoglobulin, buffered aqueous solution
Synonym: HOX4; HOX4G; Hox-4.7
Product Type: Chemical
Western Blotting Western Blot analysis of HOXD1 expression in transfected 293T cell line by HOXD1 monoclonal antibody, clone 4F4. Lanes Lane 1: HOXD1 transfected lysate (34.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (35.64 kDa).
Immunoprecipitation Immunoprecipitation of HOXD1 transfected lysate using anti-HOXD1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with HOXD1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged HOXD1 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~36.01 kDa |
| NCBI accession no. | NM_024501   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9GZZ0 |
| General description: | This gene is a member of the Antp homeobox family and encodes a protein with a homeobox DNA-binding domain. This nuclear protein functions as a sequence-specific transcription factor that is involved in differentiation and limb development. Mutations in this gene have been associated with severe developmental defects on the anterior-posterior (a-p) limb axis. (provided by RefSeq) |
| Immunogen: | HOXD1 (NP_078777, 151 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence QVDYAAFGEPGPFPACLKASADGHP |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |