Monoclonal Anti-NDUFA5 antibody produced in mouse
SIGMA/SAB1402855 - clone 4A2, purified immunoglobulin, buffered aqueous solution
Synonym: B13; CI-13KD-B; DKFZp781K1356; FLJ12147; NUFM; UQOR13
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to NDUFA5 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 μg/mL]
Western Blotting Western Blot analysis of NDUFA5 expression in transfected 293T cell line by NDUFA5 monoclonal antibody, clone 4A2. Lanes Lane 1: NDUFA5 transfected lysate (13.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting NDUFA5 monoclonal antibody, clone 4A2. Western Blot analysis of NDUFA5 expression in human kidney.
Western Blotting QC Western Blot detection against Immunogen (39.9 kDa).
ELISA Detection limit for recombinant GST tagged NDUFA5 is 10 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4A2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~40.5 kDa |
| NCBI accession no. | NM_005000   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q16718 |
| General description: | The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. (provided by RefSeq) |
| Immunogen: | NDUFA5 (NP_004991.1, 1 a.a. ~ 116 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAGVLKKTTGLVGLAVCNTPHERLR |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |